{"id":45,"date":"2012-10-08T12:49:00","date_gmt":"2012-10-08T17:49:00","guid":{"rendered":"https:\/\/skepticink.com\/smilodonsretreat\/?p=45"},"modified":"2012-10-08T12:49:00","modified_gmt":"2012-10-08T17:49:00","slug":"my-take-on-the-gmo-causes-cancer-study","status":"publish","type":"post","link":"https:\/\/skepticink.com\/smilodonsretreat\/2012\/10\/08\/my-take-on-the-gmo-causes-cancer-study\/","title":{"rendered":"My Take on the GMO Causes Cancer Study"},"content":{"rendered":"<p>I have fallen into the genetically modified organism &#8216;discussion&#8217; by accident.\u00a0 I have a friend who is violently anti-GMO.\u00a0 This friend regularly posts the lies and fake &#8216;studies&#8217; that populate the anti-GMO movement.\u00a0 I started by just looking up a few stats and now, I&#8217;m into full-blown deep research mode on this stuff.<\/p>\n<p>The research, really is quite interesting and I may go into more of it.\u00a0 But the one thing I wanted to bring up here is the concept of cherry-picking.\u00a0 Why, I keep asking my friend, do you keep bringing up non-peer reviewed studies and ignore the peer-reviewed studies that I present to you?\u00a0 The ready answer is this massive global conspiracy between Monsanto and the bought government.<\/p>\n<p>Honestly, I don&#8217;t like Monsanto and their practices.\u00a0 I&#8217;m not a huge fan of Roundup Ready plants, because that does tend to increase the use of herbicides.\u00a0 But that doesn&#8217;t mean that ALL GM food must be eliminated.<\/p>\n<p>So, finally, a study appears that seems to support the Anti-GM crowd&#8217;s claims.\u00a0 GM food causes cancer.\u00a0 Well, I was skeptical.\u00a0 Even though this paper was published in a peer-reviewed journal, it was pre-published alongside 3 articles that all concluded that GM food was not harmful.<\/p>\n<p>I contacted the lead author and got a copy of the paper, which is <a href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0278691512005637\">now available here<\/a>.<\/p>\n<p>Honestly, after reading the abstract, I could see that this might be a good paper.\u00a0 After reviewing the graphs (<a title=\"Anti-GM Food Proponents and\u00a0Research\" href=\"http:\/\/ogremk5.wordpress.com\/2012\/09\/19\/anti-gm-food-proponents-lie\/\">here<\/a>), I had some concerns.\u00a0 After reading the paper I have some major concerns.<\/p>\n<p><!--more--><\/p>\n<p>First, I want to talk a bit about the authors.\u00a0 This isn&#8217;t an <a href=\"http:\/\/en.wikipedia.org\/wiki\/Ad_hominem\">ad hominem<\/a> attack.\u00a0 If you are talking to a car salesman about which car is best and he describes the virtues of a particular car, oh and by the way I have one for sale here\u2026 perhaps you should consider the source.<\/p>\n<p>Now, the lead author, Gilles-Eric S\u00e9ralini, is also the author of a couple of books.\u00a0 One of which is title \u201cGenetically Incorrect\u201d and another is \u201cWe Can Clean Up\u201d.\u00a0 These are books talking about how genetic manipulation of organisms is wrong (ironic considering the lab rats he used for this research, but more on that in a bit) and are part of the organic food revolution in France.\u00a0 Do you honestly think he\u2019s unbiased?<\/p>\n<p>One other point.\u00a0 <a href=\"http:\/\/www.efsa.europa.eu\/de\/efsajournal\/doc\/753.pdf\">S\u00e9ralini has been censured before for incorrect analysis of data and incorrect procedures<\/a>.<\/p>\n<p>Another author, Jo\u00ebl Spiroux de Vend\u00f4mois, is a <a href=\"http:\/\/www.criigen.org\/SiteEn\/index.php?option=com_content&amp;task=view&amp;id=60&amp;Itemid=105\">homeopath<\/a>.\u00a0 I\u2019m sorry, but the fundamental principles of homeopathy (like cure like, dilution, rejection of the germ theory of disease) are all wrong.\u00a0 I\u2019ve talked briefly about this before and I\u2019ll happily go into it again if anyone wants me to.\u00a0 But the principles of homeopathy aren\u2019t even wrong, they are impossible.<\/p>\n<p>So, there\u2019s two of the authors of this paper.\u00a0 One seems to be committed to defeating genetic manipulation of organisms and the other (despite being a doctor) doesn\u2019t believe that science works.\u00a0 Now, would you buy a car from these two salesmen?<\/p>\n<p>Again, this isn\u2019t an ad hominem attack.\u00a0 These people freely admit that they think these things, but they don\u2019t state so in their paper under\u00a0 the \u201cconflict of interests\u201d section.\u00a0 That\u2019s not to say that these people can\u2019t do good science.\u00a0 We should examine the science\u2026 so let\u2019s do that.<\/p>\n<p>Here&#8217;s the results graph<\/p>\n<p><a href=\"https:\/\/skepticink.com\/smilodonsretreat\/files\/2012\/10\/1-s2.0-S0278691512005637-gr1.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-46\" src=\"https:\/\/skepticink.com\/smilodonsretreat\/files\/2012\/10\/1-s2.0-S0278691512005637-gr1.jpg\" alt=\"\" width=\"511\" height=\"580\" srcset=\"https:\/\/skepticink.com\/smilodonsretreat\/files\/2012\/10\/1-s2.0-S0278691512005637-gr1.jpg 511w, https:\/\/skepticink.com\/smilodonsretreat\/files\/2012\/10\/1-s2.0-S0278691512005637-gr1-264x300.jpg 264w\" sizes=\"auto, (max-width: 511px) 100vw, 511px\" \/><\/a><\/p>\n<blockquote><p>&nbsp;<\/p>\n<p>Fig. 1.\u00a0Mortality of rats fed GMO treated or not with Roundup, and effects of Roundup alone. Rats were fed with NK603 GM maize (with or without application of Roundup) at three different doses (11, 22, 33% in their diet: thin, medium and bold lines, respectively) compared to the substantially equivalent closest isogenic non-GM maize (control, dotted line). Roundup was administrated in drinking water at 3 increasing doses, same symbols (environmental (A), MRL in agricultural GMOs (B) and half of minimal agricultural levels (C), see Section <a id=\"ancf0005bs0010\" href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0278691512005637#s0010\">2<\/a>). Lifespan during the experiment for the control group is represented by the vertical bar\u00a0\u00b1\u00a0SEM (grey area). In bar histograms, the causes of mortality before the grey area are detailed in comparison to the controls (0). In black are represented the necessary euthanasia because of suffering in accordance with ethical rules (tumors over 25% body weight, more than 25% weight loss, hemorrhagic bleeding, etc.); and in hatched areas, spontaneous mortality.<\/p><\/blockquote>\n<p>They are reporting in the graphic on page 4 that 10 males of the group that was fed Roundup sprayed GM maize died.\u00a0 Ten animals.\u00a0 That sounds bad.\u00a0 Then we learn that there were only 10 animals in that group.\u00a0 Holy crap, that\u2019s a 100% death rate for those animals.<\/p>\n<p>Now look at the control group.\u00a0 All animals were fed non-GM maize with no pesticides.\u00a0 Seven out of 10 died.\u00a0 Holy crap!\u00a0 That\u2019s a 70% death rate.<\/p>\n<p>Wait\u2026 what?<\/p>\n<p>First let\u2019s look at the actual groups of animals in this study.<\/p>\n<ul>\n<li>10 male rats fed normal diet<\/li>\n<li>10 male rats fed 11% GM Maize, not sprayed with round-up<\/li>\n<li>10 male rats fed 22% GM Maize, not sprayed with round-up<\/li>\n<li>10 male rats fed 33% GM Maize, not sprayed with round-up<\/li>\n<li>10 male rats fed 11% GM Maize, sprayed with round-up<\/li>\n<li>10 male rats fed 22% GM Maize, sprayed with round-up<\/li>\n<li>10 male rats fed 33% GM Maize, sprayed with round-up<\/li>\n<li>10 male rats fed normal diet, but their water had environmental levels of round-up in the water<\/li>\n<li>10 male rats fed normal diet, but their water had half agricultural levels of round-up in the water<\/li>\n<li>10 male rats fed normal diet, but their water had agricultural levels of round-up in the water<\/li>\n<\/ul>\n<p>The females had similar groupings.\u00a0 So, there were 100 male rats and 100 female rats divided into 10 groupings.\u00a0 Then the control group of 10 was compared to each experimental group of 10.<\/p>\n<p>That\u2019s a major flaw in the study.\u00a0 Most people won\u2019t recognize it because it\u2019s very subtle, but there is a huge statistical problem here.\u00a0 The potential for cherry-picking is super strong here.\u00a0 That\u2019s not to say it happened, but then, the complete results of every animal are not published here.<\/p>\n<p>There are simply too many variables.\u00a0 A proper study would have been 50 male rats as a single test group and 50 male rats as a control group.\u00a0 The test should also be done using a couple of different breeds (again, more on why that\u2019s a concern in a minute).\u00a0 I have since learned that 50 animals is the standard for long-tern studies.<\/p>\n<p>Let\u2019s talk for a minute about correlation and causation.<\/p>\n<p>Correlation is the concept that two variables are moving at the same time.\u00a0 Causation is the concept that one of the variables is moving <strong>because<\/strong> of the other one.\u00a0 An example will serve.\u00a0 We note that over the last 100 years, the global temperature of the Earth has increased.\u00a0 We also note that over the same time period, that the number of pirates decreased.\u00a0 Those two numbers are correlated.\u00a0 Does anyone think that the lack of pirates is causing global warming (causation)?<br \/>\nYeah, I didn\u2019t think so.<\/p>\n<p>Scientists use statistical measures to analyze data and determine if two variables are correlated.\u00a0 There\u2019s no (that is zero) statistical analysis in this paper.\u00a0 Saying that 70% of a group died is not a statistical analysis.\u00a0 In fact, <a href=\"http:\/\/understandinguncertainty.org\/rats-and-gm\">one statistician is going to us this paper as an example of how NOT to do studies like this.<\/a><\/p>\n<p>Now, let\u2019s look at the causation.\u00a0 Did the GM, non-sprayed maize group have tumors and death?\u00a0 Did the GM, sprayed maize group have tumors and death?\u00a0 Did the normal feed, drinking water with pesticide group have\u00a0 tumors and death?<\/p>\n<p>The answer is yes to every single one of those.\u00a0 But note that I left one group out.\u00a0 The control.<\/p>\n<p>Did the control group have tumors and death.<\/p>\n<p>That\u2019s also a yes.\u00a0 In fact, if you look at the charts, out of 6 comparisons with the control group, two of the control groups had more tumors and death than the experimental groups.<\/p>\n<p>So we have to ask, what exactly is causing the tumors and deaths in the control group?\u00a0 Since the paper doesn&#8217;t say, we don&#8217;t know.\u00a0 Since we don&#8217;t know what is causing the issue in the control group, we cannot say that the same issue isn&#8217;t causing the cancer and death in the experimental groups.<\/p>\n<p>That&#8217;s the point of a well crafted scientific experiment, to eliminate all but one variable that is then manipulated by the researchers to observe the changes that happen.\u00a0 This is not a well crafted experiment.\u00a0 We can&#8217;t say that the GM food causes cancer until we can show that the cause of cancer in the control animals does not also cause cancer in the experimental animals.\u00a0 I&#8217;ll talk more about the animals in a moment and, I think, all will become clear.<\/p>\n<p>Statistically speaking, it\u2019s impossible to say that there\u2019s even any correlation between the tested chemicals and crops and the tumors and deaths.\u00a0 Why do I say that?\u00a0 Because in <strong>every single category, the rats fed the most amount of GM Maize, sprayed GM maize, and pesticide laden water had a higher survival rate than the other two test groups.<\/strong><\/p>\n<p>This is extremely significant.\u00a0 It means that something weird is going on.\u00a0 The authors \u2018conclusions\u2019 about a threshold amount of pesticide or mitigating affects of the pesticide on the chemical pathways don\u2019t hold up under this weird condition that the fewest deaths in three case and second fewest deaths in three cases were in the rats fed the most chemicals.<\/p>\n<p>That\u2019s roughly equivalent of saying that you have a better chance of survival if you get shot twice instead of shot once.<\/p>\n<p>Now, I promised a comment on the rats.\u00a0 The strain used in this experiment were all genetically engineered (by selection)* to be lab rats. These are highly inbred animals.\u00a0 The particular strain used in this test were virgin albino Sprague-Dawley rats at 5 weeks of age.<\/p>\n<p>Here\u2019s a very <a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/521452\">interesting report on the fact that 81% of Sprague-Dawley rats end up with multiple tumors after two years<\/a> (which is the length of time of the study).\u00a0 So, the study&#8217;s authors have used a strain of rats specifically designed to develop tumors and then they are shocked when the rats develop tumors.<\/p>\n<p>This leads me to another point.\u00a0 In another paper I referred to, my friend pointed out that the same strain of rats was used.\u00a0 My response was, so what?\u00a0 In the other paper, the authors were testing for the presence of GM DNA in the rats&#8217; blood and organs, not whether they were getting cancer or not.<\/p>\n<p>Finally, a comment on the nature of the methods.\u00a0 One method routinely employed in science is called the \u2018blind\u2019 or \u2018double blind\u2019 study.\u00a0 These two processes are used to ensure that there is no bias in experiments.\u00a0 To do a blind study, the person actually making the measurements is not told whether the subject is in the experimental group or in the control group.\u00a0 Why? Because of personal bias.<\/p>\n<p>If one is not told which group is test and which group is experiment, then bias, even unconscious bias is impossible.\u00a0 In this case, a researcher might decide that one tumor is different because it\u2019s in a test animal (especially considering the background of the lead researcher).<\/p>\n<p>Now, to my conclusion.<\/p>\n<p>Does this paper show that round-up and GM Maize are toxic?<br \/>\nNo.<\/p>\n<p>Do the points in this blog article mean that round-up and GM maize are safe?<br \/>\nNo.<\/p>\n<p>What it means is the state of round-up and GM maize is not changed by this poor study and we need to stick with previous studies that used the proper methodology and the proper analysis.<\/p>\n<p>This could have been a very valuable, useful study.\u00a0 But it just isn\u2019t.<\/p>\n<p>________________________<\/p>\n<p>* Keep in mind that maize is a genetically engineered product to (via artificial selection), it doesn\u2019t exist in the wild.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>I have fallen into the genetically modified organism &#8216;discussion&#8217; by accident.\u00a0 I have a friend who is violently anti-GMO.\u00a0 This&hellip;<\/p>\n","protected":false},"author":27,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[7,28,6,11],"tags":[33,31,30,32,29],"class_list":["post-45","post","type-post","status-publish","format-standard","hentry","category-biology","category-genetics","category-science","category-skepticism","tag-anti-gm","tag-cancer","tag-gm-food","tag-rats","tag-seralini"],"_links":{"self":[{"href":"https:\/\/skepticink.com\/smilodonsretreat\/wp-json\/wp\/v2\/posts\/45","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/skepticink.com\/smilodonsretreat\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/skepticink.com\/smilodonsretreat\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/skepticink.com\/smilodonsretreat\/wp-json\/wp\/v2\/users\/27"}],"replies":[{"embeddable":true,"href":"https:\/\/skepticink.com\/smilodonsretreat\/wp-json\/wp\/v2\/comments?post=45"}],"version-history":[{"count":0,"href":"https:\/\/skepticink.com\/smilodonsretreat\/wp-json\/wp\/v2\/posts\/45\/revisions"}],"wp:attachment":[{"href":"https:\/\/skepticink.com\/smilodonsretreat\/wp-json\/wp\/v2\/media?parent=45"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/skepticink.com\/smilodonsretreat\/wp-json\/wp\/v2\/categories?post=45"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/skepticink.com\/smilodonsretreat\/wp-json\/wp\/v2\/tags?post=45"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}